Upon maternal depletion of Eed, PADs are largely intact in oocytes, but their reestablishment after fertilization is compromised. PADs disassemble upon meiotic resumption from diplotene arrest but briefly reappear on the maternal genome after fertilization. PADs and inter-PAD (iPAD) regions form compartment-like structures with strong inter-domain interactions among nearby PADs. Oocytes at late stages show self-interacting, cohesin-independent compartmental domains marked by H3K27me3, therefore termed Polycomb-associating domains (PADs). Using low-input Hi-C (genome-wide chromatin conformation capture), we found that a unique chromatin organization gradually appears during mouse oocyte growth. However, the dynamics of three-dimensional chromatin structure in this process is poorly characterized. In mammals, chromatin organization undergoes drastic reorganization during oocyte development. Polycomb group proteins regulate chromatin architecture in mouse oocytes and early embryosĮxpression profiling by high throughput sequencing GEO help: Mouse over screen elements for information.
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